Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Fixering av blåssköljvätska för urincytologi: En jämförelse av ThinPrep® CytoLyt solution med fixeringslösning innehållande ättiksyra
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
Jönköping University, School of Health and Welfare, HHJ, Dep. of Natural Science and Biomedicine.
2021 (Swedish)Independent thesis Basic level (degree of Bachelor), 180 HE creditsStudent thesisAlternative title
Fixation of bladder washings for urine cytology : A comparison of ThinPrep® CytoLyt solution with fixative containing acetic acid (English)
Abstract [sv]

Urinblåsecancer är den sjunde vanligaste cancerformen i Sverige där blåssköljvätska utgör det huvudsakliga provmaterialet vid utredning. ThinPrep® är en vanligt förkommande metod som används inom vätskebaserad cytologi vid analys av blåssköljvätska. Fixering av provmaterialet kan försvåra diagnostiken på grund av förändrad morfologi samt artefakter.  Studiens syfte var att jämföra erhållet preparatinnehåll från blåssköljvätskor som fixerats med fixeringslösning innehållande ättiksyra med CytoLyt® solution. Studien ämnade även jämföra ThinPrep® filtren ”Non-GYN” och ”UroCyte”.  Studien inkluderade 90 patientprover av blåssköljvätska som fixerades med tillsats av ättiksyra samt CytoLyt®, varvid filtren ”Non-GYN” samt ”UroCyte” användes. Preparaten bedömdes av cytodiagnostiker samt cytopatolog utifrån kriterierna: bakgrundsmaterial, cellmängd samt färgbarhet. Resultatet visade att CytoLyt® i kombination med filtret ”Non-GYN” gav ett mer representativt preparat samt lämnade en god bakgrundsbild. Användandet av CytoLyt® resulterade i fler diagnostiska fördelar jämfört med fixering av ättiksyra, vilket även gynnar patienter. 

Abstract [en]

Bladder cancer is the seventh most common form of cancer in Sweden where the main source of material for investigation are bladder washings. A common method within liquid-based cytology for analysis of bladder washings is ThinPrep®. Altered morphology as well as artifacts due to fixation can complicate funkar “the diagnostics of the sample. The aim of this study was to compare the slide content obtained from bladder washings fixed with fixative containing acetic acid with CytoLyt® solution. The study also intended to compare the ThinPrep® filters "Non-GYN" and "UroCyte". A total of 90 samples of bladder washings from patients where included. The samples where fixed with the addition of acetic acid and CytoLyt®, using the "Non-GYN" and "UroCyte" filters. A cytotechnologist and a cytopathologist assessed the preparations based on the criteria background material, cell quantity and the amount of colour change. The results showed that CytoLyt® in combination with the filter "Non-GYN" gave a more representative preparation and provided a good background image. The use of CytoLyt® resulted in more diagnostic benefits compared to fixation with acetic acid, which also benefits patients.

Place, publisher, year, edition, pages
2021. , p. 32
Keywords [en]
ThinPrep®, cytology, CytoLyt®, acetic acid, bladder washings
Keywords [sv]
ThinPrep®, cytologi, CytoLyt®, ättiksyra, blåssköljvätska
National Category
Clinical Laboratory Medicine Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:hj:diva-52936ISRN: JU-HHJ-BLA-1-20210108OAI: oai:DiVA.org:hj-52936DiVA, id: diva2:1562386
Subject / course
HHJ, Biomedical Laboratory Science
Supervisors
Examiners
Available from: 2021-06-14 Created: 2021-06-08 Last updated: 2025-10-13Bibliographically approved

Open Access in DiVA

fulltext(1774 kB)723 downloads
File information
File name FULLTEXT01.pdfFile size 1774 kBChecksum SHA-512
a3e2e6e1d480e22e5cacb6c5902bf593b44ebf74b8f05e267109d2d862f0bc7db97e9985e82e1c55ea0c399c0b527611001b42cd5664c038154471207cbc5b08
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Lindgren, PernillaSöderblom, Evelina
By organisation
HHJ, Dep. of Natural Science and Biomedicine
Clinical Laboratory MedicineBiomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 724 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 506 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf