This bachelor thesis investigates the accessibility challenges faced by individuals with visual, physical, and hearing disabilities when interacting with the ticketing application of public transport, focusing on Zbiletem. Drawing on a thematic analysis methodology, data was collected through interviews and literature review. The study aligns with the Web Content Accessibility Guidelines (WCAG) and emphasises a user-centred design approach. Findings reveal significant challenges for visually impaired users due to lack of alternative text. Users with physical disabilities encountered navigation difficulties, while those with hearing impairments faced fewer challenges. The study underscores the importance of inclusive design practices and highlights the need for developers to consider diverse user needs. Recommendations include enhancing alternative text for images and textual elements to help technological tools such as VoiceOver read relevant information. Overall, this research contributes valuable insights into enhancing accessibility in public transport applications, aiming to create more inclusive digital environments for users with disabilities.